| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.175212 |
| Chromosome: | chromosome 10 |
| Location: | 3967665 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g448650 | KDG5 | Diacylglycerol kinase; (1 of 1) IPR005218 - Diacylglycerol/lipid kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTACATGGGAGGCCTTTTGCGGGCGCAGG |
| Internal bar code: | GCTAGCGCGCGAGTCCATGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 806 |
| LEAP-Seq percent confirming: | 91.0021 |
| LEAP-Seq n confirming: | 7474 |
| LEAP-Seq n nonconfirming: | 739 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGAAGAACGTGTGTGTCT |
| Suggested primer 2: | GTCTGGGTAGGCGGATGTAA |