Insertion junction: LMJ.RY0402.175238_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.175238
Chromosome:chromosome 16
Location:1360869
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g651900 CRI1,OPR129,OPR126,CRTISO OctotricoPeptide Repeat protein 129 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCACAGGATGCACCACTTCGAAGTCTGTC
Internal bar code:AGTCCGGGTTATAGCGTGGGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:195
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:56
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AGAGACAAGCCAGCAACGAT
Suggested primer 2:TCGAGCAAATGACTGAGTGG