Insertion junction: LMJ.RY0402.175246_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.175246
Chromosome:chromosome 3
Location:3956728
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g171650 HEL16 (1 of 2) PTHR18934:SF145 - ATP-DEPENDENT RNA HELICASE DHX57-RELATED; DEAD/DEAH box helicase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAAGAGGGGCGAGGGACAATTCGAGTGAG
Internal bar code:ATGGAAAATGGGCAGTGGCAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:966
LEAP-Seq percent confirming:99.6009
LEAP-Seq n confirming:3244
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTGCTGTTCAAGGAGCTG
Suggested primer 2:TAGATGCAGCACTCACCAGG