Insertion junction: LMJ.RY0402.175271_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.175271
Chromosome:chromosome 3
Location:111985
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g143987 (1 of 1) IPR011016//IPR014048 - Zinc finger, RING-CH-type // Methylated-DNA-[protein]-cysteine S-methyltransferase, DNA binding 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAAACGGGACCCGTACGACAGCGCACAGG
Internal bar code:GTTCCGGCAGTTCCTTCGTAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:790
LEAP-Seq percent confirming:99.5385
LEAP-Seq n confirming:1294
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CGCCTAAACGTATCGTGGAT
Suggested primer 2:AGTTCCTGTGGTGTTCAGGG