| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.175293 |
| Chromosome: | chromosome 14 |
| Location: | 3033446 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g628237 | intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCTTCCTGGCGTTCGTTTCTTGATCAGG |
| Internal bar code: | CGCTAGGGGCCGGCGCACTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 787 |
| LEAP-Seq percent confirming: | 99.3707 |
| LEAP-Seq n confirming: | 3632 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCCTTTGCATTACGTTTA |
| Suggested primer 2: | GTACCAAATGTGGCAGCCTT |