| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.175295 |
| Chromosome: | chromosome 3 |
| Location: | 2136165 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g157150 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACACCAGCTCCCGCCTAGGTGCCTGTG |
| Internal bar code: | GTGTGCGAGTGTGATCTGGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 218 |
| LEAP-Seq percent confirming: | 99.3447 |
| LEAP-Seq n confirming: | 758 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGTATGAAGGATTGGCAG |
| Suggested primer 2: | TTCCCGCAACAGCTAGACTT |