| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.175321 |
| Chromosome: | chromosome 9 |
| Location: | 1357983 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g398652 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAGCGCCGCGGGTTGGTAGTGGGAGTGT |
| Internal bar code: | CCGTTGCTGCCACCGTGCCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 836 |
| LEAP-Seq percent confirming: | 92.5489 |
| LEAP-Seq n confirming: | 20867 |
| LEAP-Seq n nonconfirming: | 1680 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACACATCCTTGCTTCTCG |
| Suggested primer 2: | GTGTCGGGTAGGTTTGCAGT |