| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.175324 |
| Chromosome: | chromosome 10 |
| Location: | 1707815 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g430350 | POB23 | Proteome of basal body 23 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTCTCTGCAGGGTCAAGGCAGGGGGTTG |
| Internal bar code: | TAGGAAAACGGTTACCATGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 628 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCACGGTCTCCCTTAACA |
| Suggested primer 2: | TGCTGCACCTTTTACAATGC |