| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.175330 |
| Chromosome: | chromosome 3 |
| Location: | 8068999 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g201150 | (1 of 2) 2.5.1.117 - Homogentisate solanesyltransferase / HST | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTAACAAAGCAACGAAAGCGCTATACGTA |
| Internal bar code: | CTTATCTAGAAAGGTAGTAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 738 |
| LEAP-Seq percent confirming: | 99.5927 |
| LEAP-Seq n confirming: | 489 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCAGCACCGTACTCGCTT |
| Suggested primer 2: | CAGAACACACAACCCAATGC |