| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.175331 |
| Chromosome: | chromosome 10 |
| Location: | 2069743 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433100 | OBGE1,OBGE | Factor possibly involved in assembly of the ribosomal 50S assembly; (1 of 1) K03979 - GTP-binding protein (obg) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGTACAAACACAAACGCACGCTAGGCCC |
| Internal bar code: | GTCGAGTAGGCCTCGAGAAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 85 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCAAAGTCTGCAAAACA |
| Suggested primer 2: | CTTGGGAACGTGGAGAATGT |