| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.175336 |
| Chromosome: | chromosome 13 |
| Location: | 3426240 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g587350 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCAGAAACGGCGGCGGTGGGTGCAGGCC |
| Internal bar code: | AAGGGAATGTTAAGCAGGGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1065 |
| LEAP-Seq percent confirming: | 87.0768 |
| LEAP-Seq n confirming: | 2392 |
| LEAP-Seq n nonconfirming: | 355 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCACAGGATGCCGTACCTT |
| Suggested primer 2: | ATACACGCTTGCACCTACCC |