| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.175358 |
| Chromosome: | chromosome 8 |
| Location: | 3389143 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g374400 | (1 of 1) PTHR19920//PTHR19920:SF0 - WD40 PROTEIN CIAO1 // CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTTGGTTAGGGTCGTGGGCTGCTGTGCC |
| Internal bar code: | CAATCTCGCCCGTTTTACCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 592 |
| LEAP-Seq percent confirming: | 99.3127 |
| LEAP-Seq n confirming: | 578 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCCTGCTACGGACCCTAA |
| Suggested primer 2: | CTGTGAGCCTGTCCCTTCTC |