| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.175367 |
| Chromosome: | chromosome 17 |
| Location: | 1906014 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g710300 | PHC31 | (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 31 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGAGGCATCGGCATCACGCACGCAGCCG |
| Internal bar code: | TTGACCCCTCGCTGGTTTGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1094 |
| LEAP-Seq percent confirming: | 99.5864 |
| LEAP-Seq n confirming: | 6260 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAGGGCGTCAACAATGTA |
| Suggested primer 2: | GCCTTGAAAGAAGTTGCCAG |