| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.175371 |
| Chromosome: | chromosome 1 |
| Location: | 5760139 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g041150 | (1 of 781) IPR000104 - Antifreeze protein, type I | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCAAGGAGGCCGAATGCTCGGTAAGTGC |
| Internal bar code: | ACAGTAGCGACTGCGGGTAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 712 |
| LEAP-Seq percent confirming: | 99.7926 |
| LEAP-Seq n confirming: | 3368 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATCAACAGCTGCAAGGAA |
| Suggested primer 2: | CTTGTCCCTTCTTGCTACGC |