| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.175385 |
| Chromosome: | chromosome 10 |
| Location: | 3404713 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g444400 | (1 of 2) K16458 - centrosomal protein CEP104 (CEP104) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTCTTCCTTCACACTTCCTTGCTATTCC |
| Internal bar code: | GGGTATTCCTGCCTCGCCGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 976 |
| LEAP-Seq percent confirming: | 96.5217 |
| LEAP-Seq n confirming: | 1221 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAGGACTGCTGGATGGGTG |
| Suggested primer 2: | TCGATAGACTGGTCGTGCTG |