| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.175394 |
| Chromosome: | chromosome 10 |
| Location: | 6281829 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g465000 | (1 of 8) 2.7.12.2 - Mitogen-activated protein kinase kinase / MKK | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAACAACAACAGGTTGTGACCTGATGTT |
| Internal bar code: | CGGTCGGGGCGCAAGTCTCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 821 |
| LEAP-Seq percent confirming: | 99.8505 |
| LEAP-Seq n confirming: | 1336 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGTGAGTCCCCACTGGTC |
| Suggested primer 2: | GGCTGAGGTGGTGGTACTGT |