| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.175394 |
| Chromosome: | chromosome 11 |
| Location: | 1853379 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467950 | FAP383 | Flagellar Associated Protein 383 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCACGTCAGCCAGGATGGCGCGGACCTG |
| Internal bar code: | GCGCCAAAACGGCTATCGAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 563 |
| LEAP-Seq percent confirming: | 72.4838 |
| LEAP-Seq n confirming: | 6712 |
| LEAP-Seq n nonconfirming: | 2548 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAGGGGAGGAGAGAGCAG |
| Suggested primer 2: | ACTATCCACACCCACGCTTC |