| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.175413 |
| Chromosome: | chromosome 14 |
| Location: | 1054905 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g614650 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATTATGTTTTTGGTGTTGGAGGCGTGGTT |
| Internal bar code: | ACGCTTGAGCGGAGCCGCGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 809 |
| LEAP-Seq percent confirming: | 99.6221 |
| LEAP-Seq n confirming: | 1318 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAGCTACTTTTCGCTGGG |
| Suggested primer 2: | TATGGTGCGTTAAAGGAGGC |