| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.175437 |
| Chromosome: | chromosome 9 |
| Location: | 2839868 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388700 | (1 of 2) PTHR23257//PTHR23257:SF428 - SERINE-THREONINE PROTEIN KINASE // INACTIVE SERINE/THREONINE-PROTEIN KINASE DDB_G0280131-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCTTGAGATGCTGAGCCATGGCTGCTG |
| Internal bar code: | GGATGTAAATTGCCTTCTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1182 |
| LEAP-Seq percent confirming: | 96.6667 |
| LEAP-Seq n confirming: | 522 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCATCACCCCCACCATATC |
| Suggested primer 2: | ATACTCGGTTCGTCTGGTGG |