Insertion junction: LMJ.RY0402.175440_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.175440
Chromosome:chromosome 6
Location:5406471
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g283950 LHCBM4 (1 of 6) K08912 - light-harvesting complex II chlorophyll a/b binding protein 1 (LHCB1); Light-harvesting Chlorophyll a/b binding protein of LHCII CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAAGAGGACAGGGGTCACATGAATGCAGG
Internal bar code:CTGCGGCGACCGGGGGAATGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:606
LEAP-Seq percent confirming:64.6469
LEAP-Seq n confirming:3286
LEAP-Seq n nonconfirming:1797
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:CGTAAGCCATTCTTTCGCTC
Suggested primer 2:TTGCGATGCTCCATAGAGTG