| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.175450 |
| Chromosome: | chromosome 13 |
| Location: | 323840 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g564250 | EIF3A | Eukaryotic translation initiation factor 3, subunit A | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTAACATGTGGAGGATGGAGGAGGTGGA |
| Internal bar code: | CCGACTTGGCATAGATGCTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1060 |
| LEAP-Seq percent confirming: | 99.7783 |
| LEAP-Seq n confirming: | 450 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTAGAACGTCCATACGGGG |
| Suggested primer 2: | CGGGGCATGAGTACAACTTT |