| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.175469 |
| Chromosome: | chromosome 17 |
| Location: | 5237313 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g735950 | (1 of 1) 4.1.1.97 - 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase / OHCU decarboxylase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGATGAAGCCGAACTTCTCCAGGTAGCGT |
| Internal bar code: | TCTATCGCTGCGGGTTTGACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 784 |
| LEAP-Seq percent confirming: | 99.8304 |
| LEAP-Seq n confirming: | 2354 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGGCTCTCCTTTGCGTAG |
| Suggested primer 2: | TGAATGTTGCAGTCACCCAT |