| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.175473 |
| Chromosome: | chromosome 17 |
| Location: | 5991427 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g740600 | MOT50 | Predicted protein; (1 of 1) 3.4.21.4 - Trypsin / Beta-trypsin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGACGAAGGAGAGGAGGAGGAAGAGGAG |
| Internal bar code: | ACGGTTGGACGGACTGTCTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 441 |
| LEAP-Seq percent confirming: | 99.4968 |
| LEAP-Seq n confirming: | 1384 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGGCTGTAGCTCAGGCAG |
| Suggested primer 2: | GGCAGAGAGGAGATGACAGC |