| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.175507 |
| Chromosome: | chromosome 3 |
| Location: | 4183813 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g173850 | (1 of 110) 3.4.19.12 - Ubiquitinyl hydrolase 1 / Ubiquitin thiolesterase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCACGCCGCGTGGTCTGGCCCGTGCCCGC |
| Internal bar code: | ACGGGATCCACTATTCTCGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 48 |
| LEAP-Seq percent confirming: | 84.6154 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCATGTGTTCAATGTGTGG |
| Suggested primer 2: | GTGACTTTGTGGAAGCAGCA |