Insertion junction: LMJ.RY0402.175523_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.175523
Chromosome:chromosome 11
Location:1460459
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467737 (1 of 1) PTHR18937//PTHR18937:SF160 - STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGTTGTCGTACAGCGTCACGCCGAGCCG
Internal bar code:CGCGAGCCATGAAGTGTTGAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:527
LEAP-Seq percent confirming:99.6831
LEAP-Seq n confirming:1573
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TACAGGCTCTTGCTCACCCT
Suggested primer 2:CTTTCAAGACGACGAGGAGG