| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.175524 |
| Chromosome: | chromosome 3 |
| Location: | 2799741 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g162150 | (1 of 26) IPR016177 - DNA-binding domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGTGGTTGCTGGTAGGCGCTCTCAGGCA |
| Internal bar code: | CGTGTAAACAACTGAGGTGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 216 |
| LEAP-Seq percent confirming: | 63.3962 |
| LEAP-Seq n confirming: | 168 |
| LEAP-Seq n nonconfirming: | 97 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTTGCTGTTGCTGTTGT |
| Suggested primer 2: | ATAAACGCCATGTACTCGCC |