| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.175536 |
| Chromosome: | chromosome 7 |
| Location: | 4342389 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g341250 | (1 of 100) IPR001810 - F-box domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATCGGATGAGCCAGCAATGCCGGTGGTG |
| Internal bar code: | TTGCCATAGGAGAAGCAAATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 722 |
| LEAP-Seq percent confirming: | 88.0115 |
| LEAP-Seq n confirming: | 9463 |
| LEAP-Seq n nonconfirming: | 1289 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGGTGGACGGCATTAC |
| Suggested primer 2: | GAACCACCCTCCTCCTCTTC |