Insertion junction: LMJ.RY0402.175540_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.175540
Chromosome:chromosome 1
Location:3213892
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g020141 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGCATCCCTGGCCGCGCGGAGTTGCAGG
Internal bar code:CTTTCGAAAGCGGGCGGGTTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:979
LEAP-Seq percent confirming:99.6762
LEAP-Seq n confirming:1539
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GAAAAACGCAGTATGCACGA
Suggested primer 2:GTGGGATCACGGTACTGCTT