| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.175541 |
| Chromosome: | chromosome_1 |
| Location: | 5705359 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g040650 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGGGCGGAAGGGTGTCAATCGGCAGCAGC |
| Internal bar code: | GGGTCCGCCCGGCCGCGCGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 911 |
| LEAP-Seq percent confirming: | 98.766 |
| LEAP-Seq n confirming: | 2321 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGAAGTCTTTGCCTTCTTG |
| Suggested primer 2: | GTGGCTACATCTGCTGACGA |