| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.175559 |
| Chromosome: | chromosome 4 |
| Location: | 1169528 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215750 | (1 of 1) K02180 - cell cycle arrest protein BUB3 (BUB3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAGATGAATCGTGTCTTCGGTTGTAGCT |
| Internal bar code: | TGGGCCCGGCTTGCCCCAGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 889 |
| LEAP-Seq percent confirming: | 99.8001 |
| LEAP-Seq n confirming: | 2496 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGAGCTTCTGTGAGACTG |
| Suggested primer 2: | GTCACACGCGATATTGTTGG |