| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.175600 |
| Chromosome: | chromosome_13 |
| Location: | 2495608 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g580150 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACCTCAGCAAACCGCCCGCCTCCTAACTAA |
| Internal bar code: | CGAATGGGGCATCTGGTCCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2 |
| LEAP-Seq percent confirming: | 0.449491 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 4208 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCAGACTTAACCAAACCA |
| Suggested primer 2: | TACGTTCCCAATAGCCAAGG |