| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.175608 |
| Chromosome: | chromosome 5 |
| Location: | 2726602 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236850 | NAT14 | N-acetyltransferase; (1 of 71) IPR016181 - Acyl-CoA N-acyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTTCATCAAGGTTTTATGCTGTACGATA |
| Internal bar code: | TGTACACGACCCCCCCGTGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 555 |
| LEAP-Seq percent confirming: | 99.7804 |
| LEAP-Seq n confirming: | 2726 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACAACCTCTACCGTAGCC |
| Suggested primer 2: | AGTGCAACATCATGCCACAT |