| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.175608 |
| Chromosome: | chromosome 8 |
| Location: | 4579239 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g383000 | (1 of 1) IPR000104//IPR001471//IPR016177//IPR031112 - Antifreeze protein, type I // AP2/ERF domain // DNA-binding domain // AP2-like ethylene-responsive transcription factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTGTGTGAGCGCTTCAGAGCCAAACATT |
| Internal bar code: | GATGGAACAATTGGTTACTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1098 |
| LEAP-Seq percent confirming: | 98.9696 |
| LEAP-Seq n confirming: | 9989 |
| LEAP-Seq n nonconfirming: | 104 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGTATGGATGGGATGGAG |
| Suggested primer 2: | CTACGTGTGCTGTGCATGTG |