Insertion junction: LMJ.RY0402.175653_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.175653
Chromosome:chromosome 8
Location:2395485
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g371000 (1 of 31) IPR003590 - Leucine-rich repeat, ribonuclease inhibitor subtype intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGAAACACAGGACCATAGGGCAGCCCACG
Internal bar code:GCAAGCGTAATCCGCTGACGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:238
LEAP-Seq percent confirming:99.5924
LEAP-Seq n confirming:11729
LEAP-Seq n nonconfirming:48
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CGCACTCATCACTCACCACT
Suggested primer 2:GTTGCAGATCTGTTGGAGCA