Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.175653 |
Chromosome: | chromosome_16 |
Location: | 1884363 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g655750 | TEK1 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCTCGACGCTACGAGATGCAAGCAGCCAAC |
Internal bar code: | GAGCCAACTGTCTTCGGTTTTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 335 |
LEAP-Seq percent confirming: | 99.8489 |
LEAP-Seq n confirming: | 661 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACCTCGTCCATGGTCATCTC |
Suggested primer 2: | GAGTCATGGCTGCTTGAACA |