| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.175660 |
| Chromosome: | chromosome 11 |
| Location: | 3653819 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g482450 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCACTTGGTGGGGAGAGGGGCAGGGGC |
| Internal bar code: | GTCCCTGGATGATTACGGCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 228 |
| LEAP-Seq percent confirming: | 71.7105 |
| LEAP-Seq n confirming: | 109 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTCCTCCTTGTCACTGC |
| Suggested primer 2: | CGTATGAATCAGAGACGCGA |