| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.175665 |
| Chromosome: | chromosome 9 |
| Location: | 6010422 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g403034 | (1 of 6) PF00857 - Isochorismatase family (Isochorismatase) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGCACGGGGAGTAGGCACGGGGAGTAGG |
| Internal bar code: | TCGGAATTAGGACAGGTAAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 257 |
| LEAP-Seq percent confirming: | 7.12831 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 456 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCCCTCTCAACCAATCAG |
| Suggested primer 2: | GGGCAGCACTTCGAGTTTAG |