Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.175683 |
Chromosome: | chromosome_16 |
Location: | 3525310 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g677382 | antisense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TAAGGAGCCTTCGAGCAGTGCCGAGAGCAA |
Internal bar code: | ACGGCTAACACAGTGGTGGACA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 510 |
LEAP-Seq percent confirming: | 99.6709 |
LEAP-Seq n confirming: | 2120 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCACATAGCTACAGGCACGA |
Suggested primer 2: | GTCTACAGCTGAAGGGGCAG |