| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.175700 |
| Chromosome: | chromosome 14 |
| Location: | 3957907 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g633150 | OPR66 | OctotricoPeptide Repeat protein, probably a pseudogene | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGAGCCAGCCGGTGGCCGCAACACGCAT |
| Internal bar code: | ACGAATCGACAGGACCGGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 707 |
| LEAP-Seq percent confirming: | 96.5517 |
| LEAP-Seq n confirming: | 2688 |
| LEAP-Seq n nonconfirming: | 96 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGGAAACGTTTTTCACAG |
| Suggested primer 2: | GGGGGCTTAGGACAGTTAGG |