| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.175742 |
| Chromosome: | chromosome 1 |
| Location: | 854020 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g004450 | CPLD42 | (1 of 5) PF07466 - Protein of unknown function (DUF1517) (DUF1517); conserved expressed membrane protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCACGGAGTCCAAGCACACAAAGCTCAT |
| Internal bar code: | CGGAATGGCAATGCAGCGCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1006 |
| LEAP-Seq percent confirming: | 99.4846 |
| LEAP-Seq n confirming: | 5598 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAACAGAATGCATGAGGG |
| Suggested primer 2: | TTAACTTGGGCTGCTTTGCT |