| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.175747 |
| Chromosome: | chromosome 3 |
| Location: | 3635764 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g168700 | PGP1 | (1 of 1) PTHR19288:SF33 - PHOSPHOGLYCOLATE PHOSPHATASE 2; Phosphoglycolate phosphatase/4-nitrophenylphosphatase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTTTGGGCTCGTATCTGCAACCCCGCTC |
| Internal bar code: | GGGGCAATACGGGTATAGCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 301 |
| LEAP-Seq percent confirming: | 99.0385 |
| LEAP-Seq n confirming: | 206 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCATGACTGGGGAGTAGG |
| Suggested primer 2: | AGTAGCGGGAGGGAAGAGAG |