| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.175762 |
| Chromosome: | chromosome 16 |
| Location: | 819827 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g647602 | intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTAAACCATTGCCAACCGAGAAGCCATAA |
| Internal bar code: | TAGTCAATGGCATTCTTGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 244 |
| LEAP-Seq percent confirming: | 99.5153 |
| LEAP-Seq n confirming: | 12934 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGTGCGTTTAACCTCCCT |
| Suggested primer 2: | TCGAAGAAGTGGAGCAACCT |