| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.175777 |
| Chromosome: | chromosome 4 |
| Location: | 1154946 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215800 | (1 of 2) PF08457 - Sfi1 spindle body protein (Sfi1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATTTGGAGAGGGCGAGCAGGAGGTCGGA |
| Internal bar code: | CGAAACAAGAGTCCCTTTGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 341 |
| LEAP-Seq percent confirming: | 99.6874 |
| LEAP-Seq n confirming: | 4464 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAGGAGCTGCTGACCCAGT |
| Suggested primer 2: | GACTTACACCAAGCGCACAA |