| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.175797 |
| Chromosome: | chromosome 16 |
| Location: | 7645610 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g689871 | TSI1 | (1 of 1) PTHR11946:SF82 - ISOLEUCINE--TRNA LIGASE, MITOCHONDRIAL; Putative organellar isoleucyl-tRNA synthetase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGTGAGGGGTGTAGGTGTGTTTAGATA |
| Internal bar code: | GTTGGCAGCTGGTGGAGTAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2 |
| LEAP-Seq percent confirming: | 41.2088 |
| LEAP-Seq n confirming: | 75 |
| LEAP-Seq n nonconfirming: | 107 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCAGTTCTTCAAGGTGTT |
| Suggested primer 2: | AGTTTCAGGGCAAAAGCTCA |