| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.175806 |
| Chromosome: | chromosome 4 |
| Location: | 1183661 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215600 | (1 of 1) PTHR32183:SF6 - THIOCYANATE METHYLTRANSFERASE 1-RELATED | 5'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGTCACGCCCTGGTAACCGCCCCGCCTA |
| Internal bar code: | TGATTTTTCTGATTTCTTTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 223 |
| LEAP-Seq percent confirming: | 96.4286 |
| LEAP-Seq n confirming: | 108 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAACTTTCAAGAGGCCAG |
| Suggested primer 2: | GATCTGGTGATGACGCATTG |