| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.175808 |
| Chromosome: | chromosome 2 |
| Location: | 5263293 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g106400 | PDAT1,LCA1 | Phospholipid diacylglycerol acyltransferase; (1 of 1) 2.3.1.158 - Phospholipid:diacylglycerol acyltransferase / PDAT | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGGCGTGCCAAAGTCCGTGTCTGCGCTG |
| Internal bar code: | AGTCCAGCATCGCGGCCGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 787 |
| LEAP-Seq percent confirming: | 98.89 |
| LEAP-Seq n confirming: | 980 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCACTCGTACGGAGAGAA |
| Suggested primer 2: | ACCATGTTGGAGGTCAGGAA |