Insertion junction: LMJ.RY0402.175812_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.175812
Chromosome:chromosome 6
Location:2503442
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g269000 (1 of 1) PF09493 - Tryptophan-rich protein (DUF2389) (DUF2389) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCACTGCATCCAAGCACTAAGCCGCCAGC
Internal bar code:GTCGAATGATTGTCGCGGGCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:43
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:66
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACGCCATACGAACCATAAGC
Suggested primer 2:TGGATTGGGAGCATGTGTTA