Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.175823 |
Chromosome: | chromosome 11 |
Location: | 1539502 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre11.g467752 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTGCAGTGGAACTAACGCATAGCATGTG |
Internal bar code: | CGCGCAGGATCGACCGATGAAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 627 |
LEAP-Seq percent confirming: | 99.4108 |
LEAP-Seq n confirming: | 1181 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TATGCCGCACACACATTTTT |
Suggested primer 2: | ACTGAGGCTGTGTGTGTTGC |