| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.175825 |
| Chromosome: | chromosome 3 |
| Location: | 2392497 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g158900 | RBP63,DLA2 | Dihydrolipoamide acetyltransferase; (1 of 1) PTHR23151//PTHR23151:SF61 - DIHYDROLIPOAMIDE ACETYL/SUCCINYL-TRANSFERASE-RELATED // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGTCATTTCTTCTCATGGGAAAGCGATG |
| Internal bar code: | AGGCACGCAGCATCGTCTAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 883 |
| LEAP-Seq percent confirming: | 96.7053 |
| LEAP-Seq n confirming: | 1086 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGGGTTCCCTTTTAATG |
| Suggested primer 2: | CCTTGAACCACCTCCACTGT |