| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.175853 |
| Chromosome: | chromosome 17 |
| Location: | 3823232 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g727900 | CRC1 | (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase; Centrin-related calmodulin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCCAGCGCGACAACGGCCGCTCCGCTGC |
| Internal bar code: | TGTGGTTAAACTCTGAGTAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 496 |
| LEAP-Seq percent confirming: | 99.6146 |
| LEAP-Seq n confirming: | 517 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGCTGTTGCTGTTGCTGT |
| Suggested primer 2: | TGCATTCAACAGCTGGAGTC |